Description

Align reads to a reference genome using STAR

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads{:bash}

:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

meta2{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘test’ ]

index{:bash}

:directory

STAR genome index

star

meta3{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘test’ ]

gtf{:bash}

:file

Annotation GTF file

*.{gtf}

star_ignore_sjdbgtf{:bash}

:boolean

Ignore annotation GTF file

seq_platform{:bash}

:string

Sequencing platform

seq_center{:bash}

:string

Sequencing center

Output

name:type
description
pattern

log_final{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*Log.final.out{:bash}

:file

STAR final log file

*Log.final.out

log_out{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*Log.out{:bash}

:file

STAR lot out file

*Log.out

log_progress{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*Log.progress.out{:bash}

:file

STAR log progress file

*Log.progress.out

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

bam{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*d.out.bam{:bash}

:file

Output BAM file containing read alignments

*.{bam}

bam_sorted{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}.sortedByCoord.out.bam{:bash}

:file

Sorted BAM file of read alignments (optional)

*sortedByCoord.out.bam

bam_sorted_aligned{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}.Aligned.sortedByCoord.out.bam{:bash}

:file

Sorted BAM file of read alignments (optional)

*.Aligned.sortedByCoord.out.bam

bam_transcript{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*toTranscriptome.out.bam{:bash}

:file

Output BAM file of transcriptome alignment (optional)

*toTranscriptome.out.bam

bam_unsorted{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*Aligned.unsort.out.bam{:bash}

:file

Unsorted BAM file of read alignments (optional)

*Aligned.unsort.out.bam

fastq{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*fastq.gz{:bash}

:file

Unmapped FastQ files (optional)

*fastq.gz

tab{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.tab{:bash}

:file

STAR output tab file(s) (optional)

*.tab

spl_junc_tab{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.SJ.out.tab{:bash}

:file

STAR output splice junction tab file

*.SJ.out.tab

read_per_gene_tab{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.ReadsPerGene.out.tab{:bash}

:file

STAR output read per gene tab file

*.ReadsPerGene.out.tab

junction{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.out.junction{:bash}

:file

STAR chimeric junction output file (optional)

*.out.junction

sam{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.out.sam{:bash}

:file

STAR output SAM file(s) (optional)

*.out.sam

wig{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.wig{:bash}

:file

STAR output wiggle format file(s) (optional)

*.wig

bedgraph{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bg{:bash}

:file

STAR output bedGraph format file(s) (optional)

*.bg

Tools

star
MIT

STAR is a software package for mapping DNA sequences against a large reference genome, such as the human genome.